Israel has tightened its restrictions on its only goods passage with the Gaza Strip after Palestinians launched new incendiary bombs.
Kerem Shalom will not receive fuel until Sunday, but the delivery of food and medicine is still permitted.
Israeli Defense Minister Avigdor Lieberman said he was responding to the "continuing terrorist attacks" of the Palestinian Islamist movement Hamas.
Hamas, which dominates Gaza, warned Israel of "dangerous consequences".
On Saturday, in response to some of the most intense bombing in Gaza since the Israeli-Israeli war of 2014, Israeli military raided the coastal area.
Israeli attacks killed two Palestinians and wounded 14 others. Four Israelis were wounded when more than 200 rockets and shells were fired in southern Israel.
The violence ceased after Hamas and Islamic jihad fighters accepted an Egyptian-led ceasefire.
Israeli Prime Minister Benjamin Netanyahu said he was ready to "increase the strength of our attacks" if the Palestinians do not stop launching dragons and balloons with explosive devices and explosive devices on the Israeli-Israeli border.
The devices have caused hundreds of fires in southern Israel, burning over 2,830 hectares of forests and farmland and causing hundreds of thousands of dollars of damage, according to the authorities.
The incendiary attacks began during mass demonstrations along the border, where thousands of Palestinians expressed their support for the declared right of Palestinian refugees to return to their ancestral homes in present-day Israel, and called for an end to the blockade of Palestinians. Gaza on Israel and Egypt.
According to health officials in Gaza, more than 130 Palestinians were killed and 15,000 injured by Israeli forces during the protests.
Human rights groups have accused Israeli troops of using too much force. Israel said it had opened fire only for self-defense or people who are trying to infiltrate their territory under the protection of protests.
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On Monday, Israeli aircraft bombed two Hamas posts after the bombing had caused four fires in Israel.
Later, Lieberman announced that Kerem Shalom would be closed for fuel deliveries and that the distance that the fishermen of Gaza would allow to the coast would be reduced from six nautical miles (12 km) to three.
It arrived a few days after Israel suspended imports and exports across borders, except for basic humanitarian supplies – fuel, food, some medical and medical equipment, livestock and animal feed – to put pressure on Hamas.
A Hamas spokesman described the closure as a "crime against humanity".
"These vindictive measures reflect the magnitude of the repression and the ugliness of the crime in Gaza, which will have dangerous consequences for which the occupation is fully responsible," said Fawzi Barhoum.
The Israeli NGO Gisha, which supports the freedom of movement of the Palestinians, said that "the closure of the main artery of Gaza is a horrible act of illegal collective punishment" against the two million inhabitants of Gaza, most of whom are children ".
Israel and Egypt imposed a blockade of the Gaza Strip against land, sea and air while Hamas strengthened its power in the territory in 2007, rejecting its rivals a year after winning the parliamentary elections. The two countries say the block is for self-defense.