The iconic motorized chair was used by Stephen Hawking after being paralyzed by motor neuron disease.
And tonight it was sold at auction for 296,750 pounds.
It was expected that it would sell for around £ 10,000.
The sale also included the doctoral thesis of the renowned scientist on the origins of the universe and a screenplay of one of his appearances on The Simpsons.
Diagnosed with the motor neuron disease at age 22, the disease eventually left Professor Hawking almost completely paralyzed.
He died in March at the age of 76.
Thomas Venning, a crier of Christie, said that the wheelchair has become a symbol not only of disability, but also of the "pungent sense of humor" of Professor Hawking.
Once he ran on Prince Charles's toes and reportedly joked that he wanted to do the same with former Prime Minister Margaret Thatcher.
He also appeared in a skit Monty Python that ran with his physicist Brian Cox with his wheelchair.
Hawking's thesis of 1965 on the origins of the universe was sold for 584,750 pounds, more than three times its pre-sale estimate.
Venning said the newspapers "trace the development of his thought – this brilliant electrifying intelligence".
The proceeds from the sale will go to two charities, the Stephen Hawking Foundation and the Motor Neurone Disease Association.
"It was part of his personality"
Hawking's daughter, Lucy, said the sale gave "admirers of her work the chance to capture a memory of our father's extraordinary life in the form of a small selection of evocative and fascinating objects."
Other noteworthy items put up for auction included a document signed by Isaac Newton repaying a loan, sold for 56,250 pounds, a letter from Charles Darwin described as his "brilliant review of the Challenger Expedition's naturalist report," which sold for £ 15,000, and a copy of Einstein's views on Newton, which was once owned by the former son and sold for £ 13,750.
Last month a recording made by Professor Hawking to warn of the dangers posed by Brexit and Donald Trump was broadcast on the occasion of the launch of his latest book.
In the message "Beyond the Grave" he cited the election of Trump and Britain's vote to leave the European Union as part of "a global uprising against experts, including scientists".